Evidence that superficial branchial colonies on the gills of Salmo salar L. are not Aeromonas salmonicida     

J. F. TURNBULL  R. H. RICHARDS  M. F. TATNER 《Journal of fish diseases》1989,12(5):449-458

Abstract. The indirect immunoperoxidase technique (IPT) was used to examine specific superficial colonies of organisms observed on the gills of salmonids. The evidence obtained suggested that the superficial colonies were not a form of Aeromonas salmonicida , the causative agent of furunculosis. Application of the IPT to bacterial smears and previously stained histological sections from a case of furunculosis, confirmed the sensitivity of the technique and suggested the presence of common antigens between A. salmonicida and members of the Vibrionaceae, Pseudomonaceae and Enterobacteriaceae.  相似文献   

Ultrastructural observations on the cellular response of carp, Cyprinus carpio L., to eggs of the blood fluke Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae)     

D. T. RICHARDS  D. HOOLE  J. W. LEWIS  E. EWENS  C. ARME 《Journal of fish diseases》1994,17(5):439-443

Abstract. The cellular response, within the kidney of experimentally infected carp to the presence of eggs of the blood fluke Sanguinicola inermis has been investigated using transmission electron microscopy. Initially, 0–1-wcck-old eggs were surrounded by host eosinophils which degranulated and broke down adjaeent to the egg shell. Infiltration by neutrophils and monocytes occurred around 1–2-week-old eggs. Eggs at 2–3 weeks of age began to become encapsulated, although the first signs of structural damage appeared when the eggs were 5–6 weeks of age.  相似文献   

Development of monoclonal antibodies to PK'X', the causative agent of proliferative kidney disease   总被引：1，自引：0，他引：1  

A. ADAMS  R. H. RICHARDS  M. MARIN DE  MATEO 《Journal of fish diseases》1992,15(6):515-521

Abstract. Two monoclonal antibody probes were produced against PK'X' cells. The parasites were partially purified by filtration and centrifugation of kidney tissue from rainbow trout with proliferative kidney disease and used as antigen for immunization of mice. The resulting monoclonal antibodies reacted with PK'X' cell antigens in enzyme-linked immunosorbent assay and immunohistochemistry tests. One antibody (Mab 12) appeared to be specific for PK'X'in kidney tissue, while the other (Mab 18) cross-reacted with host cell antigens in the kidney tubules. These probes are invaluable tools for the investigation of parasite surface antigens and life cycle studies.  相似文献   

Right heart catheterization; its contributions to physiology and medicine     

RICHARDS DW 《Science (New York, N.Y.)》1957,125(3259):1181-1185

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